Analysis Note

ControlHeLa cell lysate

Application

This Anti-Kindlin-2 Antibody, clone 3A3 is validated for use in WB, IC for the detection of Kindlin-2.

Research Sub CategoryCytoskeleton

Research CategoryCell Structure

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The Kindlin family is a group of focal adhesion proteins that have become a point of interest because of their ability to bind and activate integrins. Abnormalities in this ability have linked Kindlin to several diseases, both inherited and acquired, such as Kindler syndrome, cancer, and leukocyte adhesion deficiency. The isoform Kindlin-2 specifically participates in the recruitment of migfilin protein to focal adhesion sites for cell-ECM interactions, coordinates the manufacture of actin and modifications to cell shape. It is also actively involved in connecting ECM adhesion sites and the actin cytoskeleton (Shi, 2007). Kindlin-2 is pervasively expressed throughout tissue.

Immunogen

Epitope: Unknown

GST-tagged recombinant protein corresponding to human Kindlin-2.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/ml of this antibody detected Kindlin-2 in 10 µg of HeLa cell lysate.

Specificity

The antibody recognizes Kindlin-2.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 70 kDa